European Union Price (As per 01-01-2016):
1000 mL (2x 500 mL):
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HaemoScan High Intensity HRP-Chemiluminescence ELISA Substrate uses an enhanced chemiluminescence formula which results in luminescence signals up to four times the intensity of the top competitor and up to 13 times the intensity of a standard Luminol + Phenol based substrate  (Figure 1), providing high sensitivity during ELISA. The luminescence signal of this substrate reaches its maximum within minutes (Figure 2). Luminescence based ELISAs are usually performed in black or white opaque microplates, but may also be performed in tubes.
- High signal intensity (up to 13x signal intensity of a standard Luminol + Phenol based substrate )
- Rapid light generation (maximum signal is produced within minutes)
- Large dynamic range (detection down to picogram levels)
- High signal:noise ratio (low background)
- Convenient handling (easy-to-use 1:1 mixture of two components)
- Convenient storage (ambient shipping and storage conditions)
HaemoScan High Intensity HRP-Chemiluminescence ELISA Substrate is an enhanced chemiluminescence substrate for the detection of horseradish peroxidase (HRP) activity, optimized to detect picogram level proteins in ELISA.
Peroxidases such as horseradish peroxidase catalyze the oxidation of luminol to 3-aminophthalate in the presence of a catalyst such as perborate. This reaction is accompanied by the emission of light at 425 nm. Chemiluminescent based ELISA’s are quantified by using a luminometer to measure the relative light units (RLU). In contrast to colorimetric (chromogenic) substrates which produce a colored product that persists after the enzyme-substrate reaction has occured, chemiluminescence substrates only produce light during the enzyme-substrate reaction and can be measured immediately.
Storage and Stability
Product is stable at room temperature for at least one year.
 G. H. Thorpe, L. J. Kricka, S. B. Moseley, and T. P. Whitehead, “Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays.,” Clin. Chem., vol. 31, no. 8, pp. 1335–41, Aug. 1985.