Product #: K007
Hemolysis Assay for Solutes
Hemolysis test kit for Solutes (2x96 determinations)
European Union Price: €520,- (As per 01-01-2024).
Inquire for a quotation if you are located outside the European Union.
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Description
Determining hemolytic activity is a requirement when during the development process of new solutes/pharmaceuticals. This assay is based on erythrocyte lysis induced by contact, leachables, toxins, metal ions, surface charge or by any other cause. In vivo hemolysis may induce (severe) adverse events, due to anemia and the release of iron or other bioactive substances.
Application
Measuring solute induced hemolysis. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.
Principle
An erythrocyte suspension is incubated with the solute at 37ºC for 1 hour. After incubation the samples are centrifuged to obtain supernatant. The free hemoglobin concentration in the supernatant is measured by means of a spectrophotometer. A positive reference (Triton X-100) is included in the kit. The test kit can be used to screen solutes/pharmaceuticals at their highest intended concentration. Subsequently, when certain compounds are found to induce hemolysis, the test kit can be used to find the EC50 by preparing a dose-response curve.
Storage and Stability
Product is stable at 4 °C for at least six weeks.