Product #: K005

Complement Convertase Assay Test kit

Measurement of complement convertase is a sensitive and specific method to determine complement activation by biomaterials.

CCA kit (30 determinations)

European Union Price: €520,- (As per 01-01-2024).
Inquire for a quotation if you are located outside the European Union.


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Complement Convertase Assay Kit
Description

Measurement of complement convertase is a sensitive and specific method to determine complement activation by biomaterials. During incubation with plasma, complement factors can bind to the surface of the material followed by the formation of a complement convertase complex. After washing, to remove unbound complement proteins, the biomaterials are incubated in a medium with a specific chromogenic substrate. Cleavage of the substrate is quantified by measuring the optical density at 405 nm. Positive and negative controls are included in the kit. This method can be used to evaluate the biocompatibility of biomaterials and medical devices according to the international standard ISO 10993-4.

Application

Measuring biomaterial induced complement activation. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.

Principle

A test material of interest (ideally the surface area should be known) is incubated with plasma. During incubation, complement factors can bind to the surface of the material followed by the formation of a complement convertase complex. After incubation, samples are washed and analyzed for complement convertase activity with a complement convertase-specific chromogenic substrate. The rate of color development depends on how much convertase has been generated on the surface of the biomaterial.

Storage and Stability

Product is stable at -20 °C for at least six months.

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Manual / SDS

References that used the CCA assay:

  1. Manivasagam VK, Popat KC. Improved Hemocompatibility on Superhemophobic Micro-Nano-Structured Titanium Surfaces. Bioengineering. 2022 Dec 29;10(1):43.

  2. Fernández-Colino A, Wolf F, Rütten S, Schmitz-Rode T, Rodríguez-Cabello JC, Jockenhoevel S, Mela P. Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering. Front Bioeng Biotechnol. 2019 Nov 19;7:340.

  3. Wiegand C, Abel M, Hipler UC, Elsner P, Zieger M, Kurz J, Wendel HP, Stoppelkamp S. Hemostatic wound dressings: Predicting their effects by in vitro tests. J Biomater Appl. 2019 Apr;33(9):1285-1297.

  4. Naso F, Stefanelli U, Buratto E, Lazzari G, Perota A, Galli C, Gandaglia A. Alpha-Gal Inactivated Heart Valve Bioprostheses Exhibit an Anti-Calcification Propensity Similar to Knockout Tissues. Tissue Eng Part A. 2017 Oct;23(19-20):1181-1195.

  5. Aguiari P, Iop L, Favaretto F, Fidalgo CM, Naso F, Milan G, Vindigni V, Spina M, Bassetto F, Bagno A, Vettor R, Gerosa G. In vitro comparative assessment of decellularized bovine pericardial patches and commercial bioprosthetic heart valves. Biomed Mater. 2017 Feb 3;12(1):015021.

  6. Diaz-Gomez L, Alvarez-Lorenzo C, Concheiro A, Silva M, Dominguez F, Sheikh FA, Cantu T, Desai R, Garcia VL, Macossay J. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation. Mater Sci Eng C Mater Biol Appl. 2014 Jul 1;40:180-8.

  7. Gladitz M, Bauer J, Brückner P, Reinemann S, Wiegand C, Zieger M, Reddersen K, Hipler U, Frant M, Liefeith K, Radusch H. Antibacterial polyamides based on a dendritic zinc-hybrid with good biocompatibility showing reduced biofilm formation, BioNanoMaterials, 2014, 15(1-2), 31-46.

  8. Jovanovic D, et al. Polyacylurethanes as novel degradable cell carrier materials for tissue engineering. Materials, 2011, 4.10: 1705-1727.

  9. Jovanovic D, Engels GE, Plantinga JA, Bruinsma M, van Oeveren W, Schouten AJ, van Luyn MJ, Harmsen MC. Novel polyurethanes with interconnected porous structure induce in vivo tissue remodeling and accompanied vascularization. J Biomed Mater Res A. 2010 Oct;95(1):198-208.

  10. Yoshizaki T, Tabuchi N, van Oeveren W, Shibamiya A, Koyama T, Sunamori M. PMEA polymer-coated PVC tubing maintains anti-thrombogenic properties during in vitro whole blood circulation. Int J Artif Organs. 2005 Aug;28(8):834-40.