HaemoScan

Hemolysis Assay for Solutes

2024-01-04T00:00:00+01:00

Hemolysis test kit for Solutes (2x96 determinations)

European Union Price: €565,- (As per 01-01-2026).
Inquire for a quotation if you are located outside the European Union.

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Description

Determining hemolytic activity is a requirement when during the development process of new solutes/pharmaceuticals. This assay is based on erythrocyte lysis induced by contact, leachables, toxins, metal ions, surface charge or by any other cause. In vivo hemolysis may induce (severe) adverse events, due to anemia and the release of iron or other bioactive substances.

Application

Measuring solute induced hemolysis. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.

Principle

An erythrocyte suspension is incubated with the solute at 37ºC for 1 hour. After incubation the samples are centrifuged to obtain supernatant. The free hemoglobin concentration in the supernatant is measured by means of a spectrophotometer. A positive reference (Triton X-100) is included in the kit. The test kit can be used to screen solutes/pharmaceuticals at their highest intended concentration. Subsequently, when certain compounds are found to induce hemolysis, the test kit can be used to find the EC50 by preparing a dose-response curve.

Storage and Stability

Product is stable at 4 °C for at least six weeks.

Download

Manual / SDS

Video: in vitro hemocompatibility testing with the Haemobile

2022-03-02T00:00:00+01:00

In the video below we show our method for in in vitro hemocompatibility testing using our own Haemobile model. Subsequent analysis on the test objects after circulation are not shown.

Hemocompatibility testing wiht the Haemobile.

Optimal incubation time for in vitro hemocompatibility testing

2019-02-11T00:00:00+01:00

A long-standing question in in vitro hemocompatibility research is how long should an experiment continue to find useful results? We have also received this question quite often from our customers. In an effort to answer this, we compared a low and a high activating reference material in our own in vitro circulation model (Haemobile). After circulation we evaluated the degree of thrombogenicity, platelet function, inflammatory response, coagulation, and hemolysis. We concluded that 60 minutes was the optimal incubation time to discriminate between the low and high activating reference materials.

The publication can be found via the link below:

The optimal incubation time for in vitro hemocompatibility testing: Assessment using polymer reference materials under pulsatile flow with physiological wall shear stress conditions

AMI Conference: Medical Tubing & Medical Fluid Bags

2018-10-15T00:00:00+02:00

The event offered the medical fluid containment industry the chance to look at the latest advances in polymers, additives and compounds, which are driving more complex product designs including choices and plastics options, film extrusion technologies, plus more complex and competitive bag production techniques (2-5 October 2018, Woburn, MA, United States). This year’s conference was build upon the most pressing issues, taking a deeper dive into topics focusing on new advances in fluid containment supply chains to improve patient care, the latest performance materials, selection criteria, security and customization in competitive production processes.

HaemoScan was invited by Applied Market Information Ltd to speak about haemocompatibility and thrombogenicity testing necessary for Medical Tubing & Medical Fluid Bags.

More information about the conferences can be found here:

AMI Medical Tubing Conference / AMI Medical Fluid Bags Conference

New Website

2018-02-26T00:00:00+01:00

Our previous website was outdated and not very compatible with mobile devices. We are proud to present the current, new and improved website with a responsive design and up-to-date information!

Haemobile - in vitro blood flow model

2018-02-25T00:00:00+01:00

To assess hemocompatibility, blood flow models are often used and can either consist of in vivo animal models or in vitro blood flow models. Given the disadvantages of animal models, such as higher costs, more variability, more time consuming, and insensitivity due to overwhelming short-term effects of tissue injury, in vitro blood flow models are more attractive. We have developed our own in vitro blood flow model (1) which offers several important advantages:

Use of fresh human blood (within 30 minutes after blood withdrawal).
Pulsatile flow with physiological (wall) shear stress.
The model has a low background for thrombosis.
Low concentration of anticoagulatns, typically 1.5 IU/mL Heparin.
No blood-air interaction (as is the case in the Chandler model).

Another big advantage is the limited blood volume necessary per test loop, typically 3-5 mL. This enables the use of paired test samples, i.e., using blood from the same donor for test and control samples. This, in turn, minimizes the variance between donors, increasing contrast between hemocompatible and -incompatible materials and thus increasing the reliability of hemocompatibility testing.

Principle

The principal of generating pulsatile flow inside a test loop is based on angular momentum. Blood inside a polyvinyl chloride test loop containing a unidirectional check valve (Fig. 1b) is exposed to an angular acceleration. The check valve functions as a heart valve, i.e., preventing backward flow of blood, and is also necessary to accelerate the blood as well as the test loop. Following acceleration, the test loop is decelerated again till a standstill. The angular momentum of the blood results in blood flowing relatively to the polyvinyl chloride test loop and is not hindered by the valve but only by shear forces. During this period of blood flow, the test loop is returned to its starting position. By repeating the process, a pulsatile flow is created. The video below clearly illustrates the generated flow inside a test loop.

Figure 1. (a) Haemobile, the plateau on top is capable of carrying several test loops simultaneously. (b) Test loop of medical grade polyvinyl chloride tubing with an internal diameter of 3mm. A typical test loop contains approximately 3 ml of blood. (c) Close-up of the test loop containing a deployed drug eluting coronary stent.

Video 1. Principle of generating pulsatile flow inside a test loop. The test loop is filled with a colored fluid and contains air bubbles to demonstrate the pulsatile flow.

Flow and (wall) shear stress

The main innovations of our model are pulsatile flow and controlled (wall) shear stress. We have characterized the hemodynamics of our model by performing Doppler flow measurements and calculating the corresponding velocity profiles and (wall) shear stress profiles (Fig. 2). By controlling the settings of the Haemobile, the desired velocity and (wall) shear stress profile can be created. In Fig. 2D the flow of a coronary artery is simulated.

Figure 2. Mechanical validation of the Haemobile. On the left hand side, simulation of one repetition of the Haemobile with the following settings: angle of rotation = 180º; clockwise angular velocity = 720º/s; anticlockwise angular velocity = 360º/s; angular acceleration/deceleration = 3600º/s² (fixed setting). (a) Angle vs time, (b) angular velocity vs time, and (c) angular acceleration/deceleration vs time. On the right hand side, ensemble average of the flow measurement and calculation of the velocity profile and shear stress of a test loop with an internal diameter of 3mm and with the same settings as mentioned above. (d) Ensemble average of the measured flow (solid blue line) and typical coronary blood flow (dashed red line) (Ref. 23), (e) velocity across the diameter in time, and (f) shear stress across the diameter in time.

Custom test chambers

Of course not every medical device fits easily inside a test loop. To test devices with more complicated geometries, we can design custom test chambers so that the Haemobile can still be used for hemocompatibility testing. Figure 3 shows several examples of custom designed test chambers.

Figure 3. Custom designed test chambers. (a) Welded test chamber of different sized PVC tubing containing an impeller of an artificial heart. (b) Milled polycarbonate test chamber containing an impeller of an artificial heart. (c) Exploded view of a test chamber for biological heart valves, including a semipermeable membrane (white sheet) allowing for oxygen and carbon dioxide exchange. (d) Realization of the test chamber for biological heart valves. The complete setup can be mounted on top of the Haemobile.

If you would like to make use of our services or you would like to use the Haemobile at your own facility, please contact us.

References
(1) Engels GE, Blok SLJ, van Oeveren W. In vitro blood flow model with physiological wall shear stress for hemocompatibility testing - An example of coronary stent testing. Biointerphases 2016;11:31004.

Our AP50 and CH50 test kits have been improved

2018-02-25T00:00:00+01:00

Continuous development of our AP50 and CH50 test kits has let to an improved shelf life, from four to six weeks! In addition, the quality of the preserved red blood cells has improved.

ISO 9001:2015 – Successful transition audit

2018-02-24T00:00:00+01:00

In December 2017 the quality management system of HaemoScan was audited by DNV-GL and was found to be in compliance with the new ISO 9001:2015 standard. In the next period, we will adjust and improve the quality management system even further so that we may better serve our stakeholders.

You can download a copy of our certificate here.

R&D grant by SNN and Province Groningen

2018-02-23T00:00:00+01:00

The goal of this project is to further research and develop the design of a quantitative platelet function test (PFT) and validate it for point-of-care-test (POCT) use, and to screen and monitor patients at risk of either thrombosis or excessive bleeding. Despite the evident need to monitor platelet function in several large patient groups, there is no widely accepted quantitative PFT available. Futhermore, current tests are not sufficiently accurate to deliver quantitative results or are complicated in their use.

Distributors

2018-02-23T00:00:00+01:00

Australia & New Zealand

Abacus dx

9 University Drive
Meadowbrook, QLD, 4131
Australia
Tel: +617 3386 7999
Fax: +617 3386 7900
Email: orders@abacusdx.com
Website: www.abacusdx.com

China

Shanghai Bioleaf Biotech Co., Ltd

Rm C801,1-7#,1356 Lane
Xinyuan Rd,Minhang Dist.
Shanghai, 201100
People's Republic of China
Tel: (86)-21-65538622
Fax: (86)-21-65538480
Email: info@bioleaf.com
Website: www.bioleaf.com

Japan

Filgen Inc.

1-1409, Jonoyama, Midori-ku, Nagoya, Aichi-pref.
459-8011, Japan
Tel: +81-52-624-4388
Fax: +81-52-624-4389
Email: support@filgen.jp
Website: filgen.jp

South Africa

Reliable Diagnostic Supplies (Pty) Ltd

4 Meyer Road Edenvale 1609
South Africa
Tel: +27 10 223 7461
Email: info@rdsupplies.co.za
Website: rdsupplies.co.za

HaemoScan

Hemolysis Assay for Solutes

Hemolysis test kit for Solutes (2x96 determinations)

Description

Application

Principle

Storage and Stability

Download

Video: in vitro hemocompatibility testing with the Haemobile

Optimal incubation time for in vitro hemocompatibility testing

AMI Conference: Medical Tubing & Medical Fluid Bags

New Website

Haemobile - in vitro blood flow model

Principle

Flow and (wall) shear stress

Custom test chambers

Our AP50 and CH50 test kits have been improved

ISO 9001:2015 – Successful transition audit

R&D grant by SNN and Province Groningen

Distributors

Australia & New Zealand

Abacus dx

China

Shanghai Bioleaf Biotech Co., Ltd

Japan

Filgen Inc.

South Africa

Reliable Diagnostic Supplies (Pty) Ltd

South Korea

Kim & Friends, Inc.