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N-acetyl-glucoseaminidase (NAG) is excreted into the urine following injury to the tubular system of the kidney. This injury may occur after renal ischemia or may be induced by an inflammatory reaction. A quantitative measure of tubular damage can be obtained after simultaneous measurement of urea, to correct for dilution. NAG is determined by means of a substrate conversion assay. Under appropriate conditions, NAG present in a urine sample converts a chromogenic substrate. After development of the color the optical density is determined. The concentration [U/L] is quantified by applying standards with known concentrations of the enzyme.
Measuring N-acetyl-glucosaminidase activity. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.
The enzyme N-acetyl-glucosaminidase (NAG) can be used as a marker for kidney proximal tubulus damage. The samples are incubated with a chromogenic substrate. After one hour of incubation at low pH, a basic stop solution is added and the optical density at 400 nm is measured. Because the samples may contain components which can result in a high background signal, for every sample a blank value is determined in which the stop solution is added prior to the reaction, instead of after the reaction. The concentration of NAG is expressed in units/liter [U/L]. One unit of enzyme can convert 1 mmol of substrate per minute at a pH of 4,25 and a temperature of 25°C.
Storage and Stability
Product is stable at -20 °C for one year.
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