Fibrinolysis

To prevent excessive clotting and occlusion of major blood vessels (thrombosis), fibrin is redissolved (fibrinolysis) and inhibitory factors are activated as soon as vessel repair is initiated. Plasmin is the major enzyme responsible for degrading the Fibrin mesh network into soluble fibrinopeptides.

 

For measuring the extent of fibrinolysis we offer the following assays:

 

D-Dimer

D-dimer is a product of plasmin-mediated degradation of cross-linked fibrin. Therefore, it is considered a biochemical marker for thrombus dissolution and an indicator for fibrinolytic status. D-dimer levels can be determined in plasma by means of an enzyme immunoassay.

 

Fibrinogen degradation products

The FDP assay measures amounts of the fibrin and fibrinogen split products inblood and directly indicates the level of activityof the fibrinolytic system. High levels of FDP will indicate increased fibrinolysis. Excessive fibrin degradation products are released into the plasma in three main conditions: disseminated intravascular coagulation (DIC), thromboembolytic therapy, and primary fibrinogenolysis. Fragments X, Y, E, and D are released whenever fibrin or fibrinogen is broken down by plasmin. This degradation occurs in all three situations.

 

Plasminogen activator inhibitor-1 (PAI-1)

Plasminogen activator inhibitor-1 is a serine protease inhibitor that functions as the principal inhibitor of tissue plasminogen activator.

 

Tissue plasminogen activator (tPA)

Tissue plasminogen activator (tPA) is a serine protease found on endothelial cells, it is an enzyme that catalyzes the conversion of plasminogen into plasmin.