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Complement hemolytic activity is a functional test of the classical and alternative pathway of complement in plasma or serum. The alternative pathway method (AP50) is based on lysis of sensibilized rabbit erythrocytes in the presence of Mg++. This method is suited to evaluate the haemocompatibility of biomaterials and medical devices according to the international standard ISO 10993-4:2002 after blood, plasma, or serum contact with biomaterials and to assess the effects of pharmaceuticals on inhibition or consumption of complement components.
Measuring alternative pathway complement activity. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.
An erythrocyte suspension is incubated for 30 minutes with serial diluted serum or plasma at 37 ºC. After incubation samples are centrifuged to obtain supernatant, containing free hemoglobin. The hemoglobin concentration is measured by means of a spectrophotometer. Positive reference is total lysis induced by lysis fluid, negative reference is obtained after incubation with buffer. The kit is designed to determine hemolytic activity of small samples (50 µL or less) and can be performed in a 96 well microtiter plate.
Storage and Stability
Product is stable at 4 °C for at least one month.